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                                                              當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 細(xì)胞生物學(xué)試劑 > IL-10細(xì)胞因子
                                                              IL-10細(xì)胞因子
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                                                              產(chǎn)地/品牌:廈門/逸漠IMMOCELL產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:10ug/瓶 最后更新:2024-2-20
                                                              貨       號:IMF-I10
                                                              CAS   號:
                                                              參考報價:1200
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                                                              ▍Backgound

                                                              Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3.

                                                              ▍Source

                                                              Recombinant Human IL-10 is expressed from Escherichia coli. It contains AA Ser 19 - Asn 178. Predicted molecular mass 21.4 kDa. This protein carries a polyhistidine tag at the N-terminus.

                                                              Recombinant Human IL-10 is expressed from Escherichia coli

                                                              ▍Endotoxin

                                                              Less than 1.0 EU per μg by the LAL method.

                                                              ▍AA Sequence

                                                              SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN

                                                              ▍Purity

                                                              Purity >90% as determined by SDS-PAGE.

                                                              ▍Formulation

                                                              Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

                                                              ▍Reconstitution

                                                              Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

                                                              ▍Storage

                                                              For long term storage, the product should be stored at lyophilized state at -20°C or lower.

                                                              Please avoid repeated freeze-thaw cycles.

                                                              This product is stable after storage at:

                                                              -20°C to -70°C for 12 months in lyophilized state;

                                                              -70°C for 3 months under sterile conditions after reconstitution.

                                                              ▍SDS-PAGE

                                                              Human IL-10, His Tag on SDS-PAGE under reducing (R) condition

                                                              Human IL-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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