▍Backgound

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3.

▍Source

Recombinant Human IL-10 is expressed from Escherichia coli. It contains AA Ser 19 - Asn 178. Predicted molecular mass 21.4 kDa. This protein carries a polyhistidine tag at the N-terminus.

Recombinant Human IL-10 is expressed from Escherichia coli

▍Endotoxin

Less than 1.0 EU per μg by the LAL method.

▍AA Sequence

SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN

▍Purity

Purity >90% as determined by SDS-PAGE.

▍Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

▍Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

▍Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

▍SDS-PAGE

Human IL-10, His Tag on SDS-PAGE under reducing (R) condition

Human IL-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

廈門逸漠生物科技有限公司(Xiamen Immocell Biotechnology Co.,Ltd.)坐落于廈門國家留學創(chuàng)業(yè)園(翔安)產(chǎn)業(yè)園，是一家專注于細胞生物學領域新產(chǎn)品、新技術開發(fā)的高新技術企業(yè)。
公司擁有一支具有深厚造詣和豐富經(jīng)驗的高端技術人才，建設了高規(guī)格的研發(fā)實驗室與生產(chǎn)車間，建立了規(guī)范的研發(fā)、生產(chǎn)、銷售、客服管理體系。
公司與國內(nèi)外多家醫(yī)院、高等院校、科研院所、生物醫(yī)藥公司建立了良好的合作關系，贏得了良好的市場聲譽和廣泛的客戶認可，將努力成為生命科學界可靠的合作伙伴和堅強的技術后盾。
企業(yè)文化我們的使命：
深耕細胞技術，共筑人類健康從細胞技術著手，深入地、多維度地進行探索與積累,發(fā)展新產(chǎn)品與、新技術服務于生命科技探索以及人類疾病診療、人類健康發(fā)展事業(yè)。
我們的愿景：
成為生命科學領域被認可與喜歡的公司我們旨在構建細胞資源與應用中心。我們追求為客戶提供靠譜的產(chǎn)品與技術，讓他們能夠省心省力的專注于自己的科研工作。我們的愿景是讓客戶獲得成功的同時。成為他們的心中認可與喜歡的公司。
我們的經(jīng)營理念：
1.精益求精、開拓創(chuàng)新把成熟的產(chǎn)品做到非凡，把創(chuàng)新的產(chǎn)品做到超乎想象。
2.專業(yè)高效、合作共贏專業(yè)就是力量，專業(yè)才能高效;合作就會發(fā)展、合作才能共贏。我們秉持這種理念，以我們專業(yè)的產(chǎn)品和服務，與廣大合作伙伴共同應對挑戰(zhàn),共同發(fā)展進步。
我們的價值觀：
1.勇于探索逸漠的精髓基于勇敢地探索，于逆境中的自我激勵、奮然而起，于順境中自我挑戰(zhàn)、自我超越。逸漠人選擇創(chuàng)造更多的價值，選擇擁有精彩的人生。
2.團結奮進面對神奇的世界、美好的未來，我們心懷敬畏與謙卑。我們改變自己,適應時代，我們團結一致、凝聚力量，努力學習、不斷進步，一起展現(xiàn)我們的創(chuàng)造力與影響力。
3.守護誠信逸漠發(fā)展的過程就是不斷被別人認可的過程。我們忠誠于自己的事業(yè)，守信于他人的事務，這是我們的寶貴的財富、強大的基石。我們建立誠信、珍惜誠信，可以讓我們更加專注于處理自己的事情，也讓事情處理起來更加簡單、高效。
4.關愛生命尊重與關愛生命，是我們職責，也是我們的發(fā)展動力。我們守護自己與家人，快樂工作，享受生活;我們珍視生存的世界，崇尚和諧、友善的人際關系;熱愛優(yōu)美、多彩的人居環(huán)境。