Description:

Rabbit polyclonal antibody

  Background:

Two distinct ferredoxin-NADP(+)-oxidoreductase (FNR) isoforms were found in chloroplasts of Arabidopsis thaliana, FNR-1 (AT5G66190) and FNR-2 (AT1G20020). The FNR proteins are present in both chloroplast stroma and thylakoid membranes in chloroplasts but are more abundant in the stroma.

  Synonyms:

FNR2, LFNR2, AtLFNR2, FNR-2, Leaf FNR 2

  Immunogen:

KLH-conjugated synthetic peptide of FNR2 derived from Arabidopsis thaliana AT1G20020.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

41 / 35 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

The sequence of the synthetic peptide used for immunization is 73% homologues with the sequence in FNR1 (AT5G66190).
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 2.75 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 5.5 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 3: 11 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.