Description:

Rabbit polyclonal antibody

  Background:

The light-harvesting chlorophyll a/b-binding proteins of photosystem II (LHCII) are the major components of the photosynthetic machinery in plants which contain more than 60% of plant chlorophyll.
The LHCII proteins can be grouped into six subfamilies (Lhcb1-6) which are encoded by LHC gene family, Lhcb1, Lhcb2 and Lhcb3 are the major pigment-binding proteins which are encoded by Lhcb1, Lhcb2 and Lhcb3 genes, respectively. Lhcb1, Lhcb2 and Lhcb3 polypeptides each with about 232 amino acid residues are similar in sequence, Lhcb1-3 precursors are synthesized in cytoplasm and following transport into chloroplasts inserted into thylakoid membranes structure and function. Lhcb1 and Lhcb2 are the most abundant proteins in the light harvesting antenna complex.

  Synonyms:

Lhcb/ Lhcb1;2;3

  Immunogen:

KLH-conjugated synthetic peptide of Lhcb Lhcb1;2;3 derived from Arabidopsis thaliana AT1G29910, AT2G05100, AT5G54270.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

28 / 26 kDa (AT1G29910), 29 / 26 kDa (AT2G05100/ AT5G54270)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Solanum tuberosum, Solanum lycopersicum, Spinacia oleracea, Zea mays, Triticum aestivum.
For more species homologues information, please contact tech support at tech@phytoab.com.

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.