Description:

Rabbit polyclonal antibody

  Background:

LPA1 is a chloroplast protein that contains two tetratricopeptide repeat domains and is an intrinsic membrane protein but not an integral subunit of PSII. In Arabidopsis, LPA1 interacts with PSII reaction center protein D1 and participates in the assembly of PSII.

  Synonyms:

LPA1, LOW PSII ACCUMULATION1

  Immunogen:

Recombinant protein of LPA1 derived from Arabidopsis thaliana AT1G02910.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

51 / 41 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

1/4WT: Thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 0.625 μg of chlorophyll. 
1/2WT: Thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 1.25 μg of chlorophyll. 
WT: Thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 2.5 μg of chlorophyll. 
lpa1: Thylakoid membrane protein from mutant of Arabidopsis thaliana leaf containing 2.5 μg of chlorophyll. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.