Description:

Rabbit polyclonal antibody

  Background:

PsbTc subunit is encoded by the chloroplast genome and having a molecular mass of about 3.8 kDa. PsbTc can be identified as a low molecular mass component of the isolated CP47-RC subcomplex. Deletion of the psbT (c) gene results in increased sensitivity to photoinhibitory stress.

  Synonyms:

PsbTc, YCF8

  Immunogen:

KLH-conjugated synthetic peptide of PsbTc derived from Arabidopsis thaliana ATCG00690.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

3.8 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus, Zea mays, Glycine max, Nicotiana tabacum, Gossypium raimondii, Oryza sativa Japonica Group, Solanum lycopersicum, Vitis vinifera, Physcomitrella patens, Medicago truncatula, Triticum aestivum.
For more species homologues information, please contact tech support at tech@phytoab.com.

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 0.5 μg and 1 μg of chlorophyll, respectively. 
3 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of PSII containing 1 μg of chlorophyll. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.