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| 銷售商： 北京啟維益成科技有限公司 | 查看該公司所有產(chǎn)品 >> |
| Catalogue Number | ENZ-511 |
| Synonyms | kallikrein-related peptidase 11, TLSP, hippostasin, Kallikrein-11, hK11, Hippostasin, Trypsin-like protease, Serine protease 20, PRSS20, MGC33060, EC 3.4.21. |
| Introduction | KLK11 is a multifunctional protease. KLK11 cleaves ''bz-Phe-Arg-4-methylcoumaryl-7-amide'', KLK11 is a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine rather than lysine residues. |
| Description | KLK11 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain having a molecular weight of 25.6kDa though on SDS-PAGE migrates at about 41kDa due to the glycosilation. The KLK11 is purified by proprietary chromatographic techniques. |
| Source | HEK293-F Cells. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The KLK11 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives. |
| Solubility | It is recommended to reconstitute the lyophilized KLK11 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized KLK11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence | ETRIIKGFEC KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV CNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN. |
| Biological Activity | KLK11 specific activity is greater than 2200 pmoles/min/μg when measure with 100uM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester) and 1 μg of activated enzyme. The reaction is carried out in a volume of 100μL containing 50mM Tris, 1M NaCl, 10mM EDTA, 0.1mM DTNB (5,5’Dithio-bis(2-nitrobenzoic acid)), pH8.5 at 37°C. Cleavage of the peptide substrate can be measured at a wavelength of 405nm and quantified using the extinction coeffiecient 13,260M-1cm-1. |
| Activation | KLK11 is activated in a reaction containing 100μg/ml Kallikrein 11, 10 μg/ml thermolysin, 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 7.5 for 15 minutes at 37°C. The thermolysin reaction is stopped by adding EDTA to a final concentration of 50mM. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
手機版：KLK11 Human
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