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TOP1 70kDa Human
英文名稱:TOP1 70kDa Human總訪問:258
國產(chǎn)/進口:進口半年訪問:4
產(chǎn)地/品牌:prospec產(chǎn)品類別:細胞生物學(xué)試劑
規(guī)       格:ENZ-073 最后更新:2024-12-5
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Catalogue Number ENZ-073
Synonyms DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.
Introduction TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.
Description Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.
Source Sf9 insect cells.
Formulation TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Immunological function Binds IgG-type human auto-antibodies.
Immunological tests Standard ELISA test (checkerboard analysis of positive/negative sera panels, including CDC international reference sera).
Applications Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody.
Coating concentration 0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity Greater than 80% as determined by SDS-PAGE.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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