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                                                              LDHB
                                                              英文名稱(chēng):LDHB總訪問(wèn):425
                                                              國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問(wèn):4
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類(lèi)別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:ENZ-279 最后更新:2024-12-5
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                                                              Catalogue Number ENZ-279
                                                              Synonyms Lactate Dehydrogenase, LDH.
                                                              Introduction Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.
                                                              A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
                                                              Description The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.
                                                              Source Escherichia Coli.
                                                              Physical Appearance Sterile lyophilized powder.
                                                              Formulation The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
                                                              Solubility It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.
                                                              Please prevent freeze-thaw cycles.
                                                              Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Biological Activity The specific activity was found to be 258 U/mg protein.
                                                              Unit Definition One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include a-hydroxyglutarate dehydrogenase activity.
                                                              Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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