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Catalogue Number | PKA-347 |
Synonyms | 14-3-3 ETA, YWHAH, YWHA1, Protein AS1, Tyr-3/Trp-5 Monooxygenase Activation Protein ETA. |
Introduction | YWHAH belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 ETA is found in plants and mammals, and there is 99% identity to the mouse, rat and bovine orthologs. YWHAH gene contains a 7 base pair repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia. 14-3-3 eta is specific to the site of joint inflammation. 14-3-3 proteins are colocalized with Lewy bodies in Parkinson disease, though there is no specific staining for the 14-3-3 eta subunit. There are 3 different isoforms types of 14-3-3: Beta, Gamma and ETA that are DAL-1/Protein 4.1B-binding proteins. |
Description | YWHAH Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 266 amino acids and having a molecular mass of 30.3 kDa. YWHAH is fused to N-Terminus His Tag and purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | Sterile filtered colorless solution. |
Formulation | YWHAH solution containing 20mM Tris pH-8. |
Stability | YWHAH Human Recombinant although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles. |
Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN. |
Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |