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                                                              Precursor Neurotrophin-3 Human Recombinant(proNT3 Human)
                                                              英文名稱:Precursor Neurotrophin-3 Human Recombinant(proNT3 Human)總訪問:419
                                                              國產(chǎn)/進口:進口半年訪問:6
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細胞生物學試劑
                                                              規(guī)       格:CYT-015 最后更新:2024-12-5
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                                                              Catalogue Number CYT-015
                                                              Synonyms

                                                              Precursor Form Neurotrophin-3, proNT-3.

                                                              Introduction proNT-3 (the precursor form of neurotrophin-3) functions through p75NTR and sortilin and induces neuronal apoptosis. In contrast, mature NT-3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. Since axon retrograde transport is crucial for neurotrophin signaling, proNT-3 also has an important role in the mechanism of retrograde signaling.
                                                              Description

                                                              proNT3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27.4kDa.
                                                              The proNT3 is purified by proprietary chromatographic techniques.

                                                              Source Escherichia Coli.
                                                              Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation

                                                              proNT3 was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 8.0 and 300mM NaCl.

                                                              Solubility

                                                              It is recommended to reconstitute the lyophilized proNT3 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

                                                              Stability Lyophilized proNT3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution proNT3 should be stored at 4°C between 2-7 days and for future use below -18°C.
                                                              Please prevent freeze-thaw cycles.
                                                              Purity Greater than 95.0% as determined by:
                                                              (a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Amino acid sequence

                                                              The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Asn-Met-Asp-Gln.

                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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