按品牌選擇
|
|
|||||||||||||||||||||||||||||||||
| [發(fā)表評(píng)論] [本類其他產(chǎn)品] [本類其他供應(yīng)商] [收藏] | ||||||||||||||||||||||||||||||||||
| 銷售商： 北京啟維益成科技有限公司 | 查看該公司所有產(chǎn)品 >> |
| Catalogue Number | CYT-472 |
| Synonyms | CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L. |
| Introduction | CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines . CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma , TNF-alpha , and IL2 . |
| Description | sCD40 Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16409 Dalton. The sCD40 is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a sterile concentrated solution (1mg/ml) with 10mM Sodium Phosphate pH=7.5. |
| Solubility | It is recommended to reconstitute the lyophilized sCD40 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized sCD40 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD154 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Arg-Gly-Asp. |
| Biological Activity | The ED50 as determined by its ability to induce MIP-1 alpha & TNF-alpha from mouse splenocytes was found to be 0.1 µg/ml corresponding to a Specific Activity of 10MIU/mg. |
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.1 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of sCD40 as a Reference Standard. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. |
手機(jī)版：sCD40 Mouse
- 中喬新舟限時(shí)促銷：買細(xì)胞就送支原體檢測(cè)試劑盒
- 華威中儀試劑盒年末限時(shí)優(yōu)惠來(lái)襲，儲(chǔ)值享好禮
- 美德聲推出新品重組細(xì)胞系用于生產(chǎn)單克隆血型試劑
- 百螢小麥胚芽凝集素探針染料8折優(yōu)惠，買即贈(zèng)鼠標(biāo)墊
- 上�；菡\(chéng)生物成為安普未來(lái)恒溫?cái)U(kuò)增試劑盒代理商
- 雙11科研狂歡促銷，逐典攜手埃澤思下單滿額雙重好禮
- MCE 第三屆細(xì)胞攝影大賽圓滿落幕，評(píng)選結(jié)果公布
- MCE教師節(jié)直播:細(xì)胞凋亡的機(jī)制、標(biāo)志物與檢測(cè)方法
- 大鼠肺泡巨噬細(xì)胞(NR8383)培養(yǎng)要點(diǎn)
- 干細(xì)胞誘導(dǎo)生成類器官的工藝要點(diǎn)淺析
- 細(xì)胞復(fù)蘇的原理、實(shí)驗(yàn)步驟及注意事項(xiàng)
- 10個(gè)獲批干細(xì)胞新藥IND及細(xì)胞療法應(yīng)用案例分享
- 寵物干細(xì)胞行業(yè)技術(shù)服務(wù)體系及在治療中的應(yīng)用
- 《人臍帶間充質(zhì)干細(xì)胞治療膝骨性關(guān)節(jié)炎臨床應(yīng)用指南》內(nèi)容及適用范圍
- 免疫B細(xì)胞的分類、活化機(jī)制、主要功能及在疾病治療中的應(yīng)用
- 自然殺傷細(xì)胞（NK細(xì)胞）的功能及培養(yǎng)流程
Copyright(C) 1998-2025 生物器材網(wǎng) 電話：021-64166852;13621656896 E-mail：info@bio-equip.com