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                                                              當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 細(xì)胞生物學(xué)試劑 > IGFBP 5 Human
                                                              



                                                              

                                                              
 
	
                                                              

		
                                                              
			
                                                              
			  
                                                              
			    
				
				IGFBP 5 Human
					
			    
				
					
					
                                                              
						
                                                              
							英文名稱(chēng)：IGFBP 5 Human總訪問(wèn)：257		
						
                                                              
						
                                                              
							國(guó)產(chǎn)/進(jìn)口：進(jìn)口半年訪問(wèn)：4 
						
                                                              
						
                                                              
							產(chǎn)地/品牌：prospec產(chǎn)品類(lèi)別：細(xì)胞生物學(xué)試劑
						
                                                              
						
                                                              
							規(guī)       格：CYT-464

	
	最后更新：2024-12-5
						
                                                              
						
                                                              
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Catalogue Number 
CYT-464 
                                                              

                                                              
Synonyms 
IGFBP-5, IBP-5, IGF-binding protein 5. 
                                                              

                                                              
Introduction 
IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. 
                                                              

                                                              
Description 
IGFBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton. 
                                                              IGFBP5 is purified by proprietary chromatographic techniques. 
                                                              

                                                              
Source 
Escherichia Coli. 
                                                              

                                                              
Physical Appearance 
Sterile Filtered White lyophilized (freeze-dried) powder. 
                                                              

                                                              
Formulation 
IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium Citrate PH 3.0. 
                                                              

                                                              
Solubility 
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 
                                                              

                                                              
Stability 
Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP 5 should be stored at 4°C between 2-7 days and for future use below 
                                                              -18°C.
                                                              For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles. 
                                                              

                                                              
Purity 
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE. 
                                                              

                                                              
Amino acid sequence 
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe. 
                                                              

                                                              
Biological Activity 
The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15ng/ml of Human IGF-II corresponding to a Specific Activity of 66,667IU/mg. 
                                                              

                                                              
Usage 
ProsSec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. 
                                                              >
                                                              bio-equip.com
                                                              

			
                                                              
			
					  
		
                                                              
		
	
                                                              

                                                              

 


                                                              
	
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