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                                                              M CSF Mouse
                                                              英文名稱:M CSF Mouse總訪問(wèn):205
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                                                              規(guī)       格:CYT-439 最后更新:2024-12-5
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                                                              Catalogue Number CYT-439
                                                              Synonyms CSF-1, Lanimostim, MCSF, M-CSF.
                                                              Introduction Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
                                                              Description Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
                                                              MCSF is purified by proprietary chromatographic techniques.
                                                              Source Escherichia Coli.
                                                              Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation The protein (1mg/ml) was lyophilized with 5mM Tris, pH 8.0 + 100mM NaCl.
                                                              Solubility It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below
                                                              -18°C.
                                                              Please prevent freeze-thaw cycles.
                                                              Amino acid sequence MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
                                                              Purity Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Biological Activity The ED50,  calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found 0.91ng/ml, corresponding to a specific activity of 1MIU/mg.
                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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