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                                                              OTOR Human
                                                              英文名稱:OTOR Human總訪問:269
                                                              國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問:3
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:CYT-582 最后更新:2024-12-5
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                                                              Catalogue Number CYT-582
                                                              Synonyms Otoraplin, Fibrocyte-derived protein, Melanoma inhibitory activity-like protein, OTOR, MIAL, FDP, MIAL1, MGC126737, MGC126739.
                                                              Introduction OTOR proteins is also known as fibrocyte-derived protein (Fdp) and Melanoma inhibitory activity-like (MIAL). Otoraplin is a member of the melanoma-inhibiting activity gene family. Otoraplin is a secreted 16 kDa globular protein that is expressed in the inner ear by periotic mesenchyme and developing and mature fibrocytes. OTOR is highly homologous to MIA/cartilage-derived retinoic acid-sensitive protein (CD-RAP), which is a cartilage-specific protein that is also expressed in malignant melanoma cells. The 111 amino acid mature human otoraplin contains 1 SH3 domain (46 – 107 amino acids) and a Tyr at position 50 that is reportedly sulfated. Otoraplin takes pasrt in the initiation of periotic mesenchyme chondrogenesis.
                                                              Otoraplin is secreted through the Golgi apparatus and plays a role in cartilage development and maintenance. A frequent polymorphism in the translation start codon of OTOR can abolish translation and may be associated with forms of deafness.
                                                              Description Otoraplin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12.7 kDa.
                                                              The OTOR is purified by proprietary chromatographic techniques.
                                                              Source Escherichia Coli.
                                                              Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation The OTOR protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM PBS pH-7.4 and 130mM NaCl.
                                                              Solubility It is recommended to reconstitute the lyophilized Otoraplin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability Lyophilized OTOR Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OTOR should be stored at 4°C between 2-7 days and for future use below -18°C.
                                                              For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles.
                                                              Purity Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Amino acid sequence VHGIFMDRLASKKLCADDECVYTISLASAQEDYNAPDCRFINVKKGQQIYVYS
                                                              KLVKENGAGEFWAGSVYGDGQDEMGVVGYFPRNLVKEQRVYQEATKEVPTT
                                                              DIDFFCE.
                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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