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                                IL 7 Human
                                英文名稱:IL 7 Human總訪問:269
                                國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問:3
                                產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                規(guī)       格:CYT-254 最后更新:2024-12-5
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                                Catalogue Number CYT-254
                                Synonyms Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
                                Introduction IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
                                Description Interleukin-7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17412 Dalton.
                                The IL-7 is purified by proprietary chromatographic techniques.
                                Source Escherichia Coli.
                                Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                Formulation Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
                                Solubility It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                Stability Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.
                                For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                Please prevent freeze-thaw cycles.
                                Purity Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
                                (b) Analysis by SDS-PAGE.
                                Amino acid sequence MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH.
                                Biological Activity The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is < 0.5ng/ml, corresponding to a Specific Activity of 2MIU/mg.
                                Protein content Protein quantitation was carried out by two independent methods
                                1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

                                2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
                                Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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