| product information
| | background | | MPK4 (Mitogen-activated protein kinase 4) is involved in cortical microtubules organization and stabilization, root hair development and is a negative regulator of systemic acquired resistance (SAR) and salicylic acid (SA) mediated response. Alternative name: MAP kinase 4.
| | immunogen | | KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MPK4, TAIR: AT4G01370, UniProt: Q39024 | | antibody format | | chicken | polyclonal | | affinity purified IgY, in PBS pH 7.4 + 0.02 % sodium azide as preservative | liquid | | | quantity | | | | storage | | store at 4°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | AS12 2633 | anti-MPK6 | MITOGEN-ACTIVATED PROTEIN KINASE 6 | | additional information | | there is no band detected by this antibody in Mpk4-2 (does not express MPK4 protein AT4G01370) and Mpk4-2 sid 2-2 mutants. Cross-reactivity wth MPK11, 12 and 5 has been ruled out. | |
application information
|
| recommended dilution | | 1 : 1000 with standard ECL (WB) |
| expected | apparent MW | | 42.9 kDa |
| confirmed reactivity | | Arabidopsis thaliana
|
| predicted reactivity | | dicots inlucing: Brassica napus, Glycine max, Gossypium mexicanum, Nicotiana atenuata, Petroselinum crispum, Solanum lycopersicum, Solanum tuberosum, Thelungiella halophila, monocots: |
| not reactive in | | no confirmed exceptions from predicted reactivity are currently known |
| additional information | | to be added when available |
| selected references | | to be added when available, antibody released in March 2013 |
application example
30 µg of total protein from Arabidopsis thaliana, ecotype Ws-0 extracted with extraction buffer (100mM Tricine, 3mM MgSO4, 3mM EDTA, 1mM DTT) were separated on12 % SDS-PAGE and blotted 1h to PVDF (Biorad). Blots were blocked with non-fat dry milk (Biorad) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:250 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-hen IgY(H&L), horse radish peroxidase conjugated) diluted to 1:0000 in non-fat dry milk (Biorad) for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL Plus Western Blotting Detection System (GE Healthcare) according to the manufacturers instructions. Exposure time was 30 seconds.
Courtesy of Dr. Anna Kozłowska-Makulska, Warsaw University of Life Sciences, Poland
bio-equip.com
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