| product information
| | background | | NtcA acts as a transcriptional activator of genes subjected to nitrogen control. | | immunogen | | KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences including P33779 | | antibody format | | rabbit | polyclonal | | affinity purified serum | lyophilized | | | quantity | | 200 µg | for reconstitution add 100 µl, of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | collection of antibodies to other cyanobacterial proteins | | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1 : 1000 with standard ECL (WB) |
| expected | apparent MW | | 25 kDa |
| confirmed reactivity | | Synechocystis sp. PCC6803
|
| predicted reactivity | | marine Synechococcus strains, Anabaena sp., Gleobacter sp., Trichodesmium sp. |
| not reactive in | | no confirmed exceptions from predicted reactivity are currently known |
| additional information | | to be added when available |
| selected references | | to be added when available, antibody released in April 2012. |
application example
5 µg of total protein from Synechocystis sp. PCC 6803 extracted with PEB were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 32 seconds.
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