product information background UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2. immunogen KLH-conjugated synthetic peptide derived from known UCP protein sequences, including UCP1 (AT3G54110) and UCP2 (AT5G58970) of Arabidopsis thaliana antibody format rabbit polyclonal affinity purified serum, in PBS pH 7.4 lyophilized quantity 200 µg for reconstitution add 200 µl, of sterile water. storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB) related products antibodies to other plant mitochondrial proteins additional information Peptide used to elicit this antibody is conserved in both isoforms, UCP1 and UCP2 of Arabidopsis thaliana.

application information
recommended dilution		1 : 2000 with standard ECL (WB)
expected | apparent MW		32 kDa
confirmed reactivity		Arabidopsis thaliana, Vicia faba (protoplasts)
predicted reactivity		dicots including: Glycine max, Medicago tribuloides, Ricinus communis, monocots including: Dracunulus vulgaris, Oryza sativa, Triticum aestivum, Saccharum officinarium (sugarcane),
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available, antibody released in May 2012.

application example

10 μg of mitochondrial fraction from Arabidopsis thaliana and 25 μg of Arabidopsis thaliana leaf extract were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 5000 anti-VDAC1 antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies from Agrisera, AS09 602 and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7).