| product information
| | background | | RACK1A is a major component of RACK1 regulatory proteins playing a major role in multiple signal transduction pathways. Synonymes: Receptor for activated C kinase 1A, WD-40 repeat auxin-dependent protein ARCA.
| | immunogen | | KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RACK1A protein sequence O24456, At1g18080 | | antibody format | | rabbit | polyclonal | | serum | lyophilized | | | quantity | | 200 µl | for reconstitution add 200 µl, of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | other antibodies to signal transduction pathway components | | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1 : 2000 with standard ECL (WB) |
| expected | apparent MW | | 35 kDa |
| confirmed reactivity | | Arabidopsis thaliana
|
| predicted reactivity | | Arabidopsis thaliana |
| not reactive in | | no confirmed exceptions from predicted reactivity are currently known |
| additional information | | to be added when available |
| selected references | | Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13. |
application example
20 µg of total protein from Arabidopsis thaliana total cell extract was separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation.Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 min.
Courtesy of Dr. Sascha Laubinger, ZMBP, Germany
bio-equip.com
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