| product information
| | background | | Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L). | | immunogen | | Purified mouse IgG (H&L) AAA51107 | | antibody format | | | goat | polyclonal, | | affinity purified IgG, | lyophilized | | | quantity | | | | storage | | Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. | | related products | | other HRP-conjugated secondary antibodies against mouse IgG (H&L)
| | additional information | | Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth. Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins. | |
application information
|
| recommended dilution | | This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot |
| selected references | | |
application example
500 femtomoles of His-tagged proteins IsiA, NifH, PsbA, PsbB and PetC were loaded per gel well in Agrisera PEB extraction buffer. Proteins were separated on 4-12 % NuPAGE PAGE Bis-Tris polycacrylamide gel (Invitrogen) and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat, anti-mouse IgG horse radish peroxidase conjugated, from Agrisera AS11 1772) diluted to 1:25 000 in 2 % ECL Advance blocking reagent for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 5 seconds.
Apparent molecular weight of recombinant proteins: IsiA - 27 kda, NifH - 34 kDa, PsbA - 30-37 kDa, PsbB - 40 kDa, PetC - 23 kDa.
bio-equip.com
北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷(xiāo)售經(jīng)驗(yàn)人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長(zhǎng)期穩(wěn)定的合作關(guān)系，已成為眾多國(guó)際知名品牌的中國(guó)總代理或一級(jí)代理商；并且為滿(mǎn)足國(guó)內(nèi)客戶(hù)的最新需求，我們也與時(shí)俱進(jìn)地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標(biāo)是把國(guó)外有特點(diǎn)的生物前沿產(chǎn)品引進(jìn)中國(guó)，讓國(guó)內(nèi)科學(xué)研究機(jī)構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。
我們代理的產(chǎn)品覆蓋細(xì)胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。
細(xì)胞生物學(xué)包含細(xì)胞培養(yǎng)，細(xì)胞遷移，細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)等。
植物學(xué)包含植物抗體，植物抗生素，植物組織培養(yǎng)試劑等。
分子生物學(xué)包含表觀(guān)遺傳試劑盒，核酸提取，轉(zhuǎn)染試劑等。
免疫學(xué)包含抗體，各類(lèi)檢測(cè)試劑盒等。
生物化學(xué)包含各類(lèi)生化試劑，化學(xué)小分子，常規(guī)試劑等。
蛋白組學(xué)包含不同種屬的重組蛋白，天然提取蛋白，細(xì)胞因子等。
生物制藥與診斷試劑研發(fā)方面的試劑包含各類(lèi)蛋白抑制劑，酶類(lèi)等。
在公司全體員工的共同努力和國(guó)外生產(chǎn)商的支持下，在業(yè)務(wù)上實(shí)現(xiàn)了迅猛發(fā)展，相繼在上海、武漢、廣州、長(zhǎng)春、石家莊、哈爾濱等重點(diǎn)城市建立了分支機(jī)構(gòu)或授權(quán)服務(wù)商，并以專(zhuān)業(yè)和優(yōu)質(zhì)的服務(wù)成為多家世界500強(qiáng)企業(yè)的中國(guó)區(qū)產(chǎn)品供應(yīng)商，以及中國(guó)科學(xué)院科研采購(gòu)平臺(tái)的中標(biāo)供貨商。2008年以后業(yè)務(wù)的迅猛發(fā)展促進(jìn)了啟維益成內(nèi)部組織架構(gòu)的不斷優(yōu)化，我們建立了自己的網(wǎng)絡(luò)銷(xiāo)售平臺(tái)，業(yè)務(wù)代表銷(xiāo)售平臺(tái)，針對(duì)科研客戶(hù)與企業(yè)客戶(hù)的不同需求，綜合網(wǎng)絡(luò)平臺(tái)和業(yè)務(wù)代表平臺(tái)的優(yōu)點(diǎn)，啟維益成創(chuàng)建了科研用戶(hù)與工業(yè)客戶(hù)兩支獨(dú)立的營(yíng)銷(xiāo)團(tuán)隊(duì)，分別配備不同的專(zhuān)業(yè)技術(shù)支持和客服人員，通過(guò)網(wǎng)絡(luò)和業(yè)務(wù)代表，力求為不同類(lèi)型的客戶(hù)提供前沿專(zhuān)業(yè)的解決方案。
公司客戶(hù)遍布中國(guó)生命科學(xué)基礎(chǔ)研究、開(kāi)發(fā)應(yīng)用、制藥、疾病診斷與控制、商品檢驗(yàn)檢疫、生物技術(shù)公司、食品安全以及農(nóng)林畜牧業(yè)等諸多領(lǐng)域。
我們追求目的是與研究機(jī)構(gòu)及合作伙伴之間誠(chéng)信而專(zhuān)業(yè)的對(duì)話(huà)，力求建立長(zhǎng)久合作。我們相信我們精心提供的高效、優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù)，會(huì)使您實(shí)驗(yàn)設(shè)計(jì)更有效，實(shí)驗(yàn)操作更簡(jiǎn)便，研究結(jié)果更可靠。
放眼未來(lái)，公司將努力實(shí)現(xiàn)更大的突破，將國(guó)外優(yōu)秀產(chǎn)品不斷引進(jìn)國(guó)內(nèi)，同時(shí)積極推動(dòng)國(guó)內(nèi)的產(chǎn)品銷(xiāo)往世界各地
瀏覽該公司同類(lèi)產(chǎn)品
