Description:

Rabbit polyclonal antibody

  Background:

In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclear encoded RNA polymerase (NEP). Transcription in plastids is also mediated by a number of nuclear-encoded factors in addition to PEP and NEP. In the insoluble RNA polymerase preparation samples, a total of 18 components named as pTACs (pTAC1 to pTAC18) were identified. pTAC2 (AT1G74850) is one of the components associated with PEP complex.

  Synonyms:

pTAC2, PDE343, PIGMENT DEFECTIVE 343, PLASTID TRANSCRIPTIONALLY ACTIVE 2, PTAC2

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal section of pTAC2 protein in Arabidopsis thaliana AT1G74850.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

96 / 75 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Sorghum bicolor, Zea mays, Setaria viridis, Oryza sativa Japonica Group, Brassica rapa, Brassica napus, Cucumis sativus, Medicago truncatula, Glycine max, Solanum lycopersicum, Nicotiana tabacum, Solanum tuberosum, Gossypium raimondii, and 80-99% homologues with the sequence in Hordeum vulgare, Triticum aestivum, Spinacia oleracea, Populus trichocarpa.
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 80 μg total chloroplast protein from Arabidopsis thaliana. 
Lane 2: 10 μl total chloroplast protein from Arabidopsis thaliana. 
Lane 3: 5 μl total chloroplast protein from Zea mays. 
Lane 4: 5 μl total chloroplast protein from Oryza sativa.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.