Description:

Rabbit polyclonal antibody

  Background:

RPS6 is a translation elongation factor EF1B/ribosomal protein S6 family protein, which binds together with S18 to 16S ribosomal RNA.

  Synonyms:

RPS6, PLASTID RIBOSOMAL PROTEIN OF THE 30S SUBUNIT 6, PRPS6

  Immunogen:

KLH-conjugated synthetic peptide of PRS6 derived from Arabidopsis thaliana AT1G64510.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

23 / 18 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Brassica napus, and 80-99% homologues with the sequence in Vitis vinifera, Populus trichocarpa, Glycine max, Gossypium raimondii, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Medicago truncatula.
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 10 μl total chloroplast protein from Arabidopsis thaliana.
Lane 2: Thylakoid membrane protein from Arabidopsis thaliana containing 2.5 μg of chlorophyll.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.