▍Backgound

Interleukin-2 is a protein that has a variety of immunologic functions, the most notable being the ability of IL-2 to promote the proliferation and maturation of activated T cells. A comparison of the amino acid sequences of human and murine IL-2 shows an approximately 60% sequence similarity, and human sequence IL-2 has been found to be active on murine cell lines. The 55 kDa IL-2 Rα is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain γc/IL-2 Rγ, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rβ and γc. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 Rα synthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of na?ve CD4+ T cells but not activated CD4+ memory lymphocytes . IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

▍Source

Recombinant Human IL-2 is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153. Predicted molecular mass 19.2 kDa. This protein carries a polyhistidine tag at the C-terminus.

Recombinant Human IL-2 is expressed from human 293 cells (HEK293)

▍Endotoxin

Less than 1.0 EU per μg by the LAL method.

▍AA Sequence

APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

▍Purity

Purity >90% as determined by SDS-PAGE.

▍Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

▍Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

▍Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

▍SDS-PAGE

Human IL-2, His Tag on SDS-PAGE under reducing (R) condition

Human IL-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

廈門逸漠生物科技有限公司(Xiamen Immocell Biotechnology Co.,Ltd.)坐落于廈門國家留學創(chuàng)業(yè)園(翔安)產(chǎn)業(yè)園，是一家專注于細胞生物學領域新產(chǎn)品、新技術開發(fā)的高新技術企業(yè)。
公司擁有一支具有深厚造詣和豐富經(jīng)驗的高端技術人才，建設了高規(guī)格的研發(fā)實驗室與生產(chǎn)車間，建立了規(guī)范的研發(fā)、生產(chǎn)、銷售、客服管理體系。
公司與國內(nèi)外多家醫(yī)院、高等院校、科研院所、生物醫(yī)藥公司建立了良好的合作關系，贏得了良好的市場聲譽和廣泛的客戶認可，將努力成為生命科學界可靠的合作伙伴和堅強的技術后盾。
企業(yè)文化我們的使命：
深耕細胞技術，共筑人類健康從細胞技術著手，深入地、多維度地進行探索與積累,發(fā)展新產(chǎn)品與、新技術服務于生命科技探索以及人類疾病診療、人類健康發(fā)展事業(yè)。
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我們的經(jīng)營理念：
1.精益求精、開拓創(chuàng)新把成熟的產(chǎn)品做到非凡，把創(chuàng)新的產(chǎn)品做到超乎想象。
2.專業(yè)高效、合作共贏專業(yè)就是力量，專業(yè)才能高效;合作就會發(fā)展、合作才能共贏。我們秉持這種理念，以我們專業(yè)的產(chǎn)品和服務，與廣大合作伙伴共同應對挑戰(zhàn),共同發(fā)展進步。
我們的價值觀：
1.勇于探索逸漠的精髓基于勇敢地探索，于逆境中的自我激勵、奮然而起，于順境中自我挑戰(zhàn)、自我超越。逸漠人選擇創(chuàng)造更多的價值，選擇擁有精彩的人生。
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3.守護誠信逸漠發(fā)展的過程就是不斷被別人認可的過程。我們忠誠于自己的事業(yè)，守信于他人的事務，這是我們的寶貴的財富、強大的基石。我們建立誠信、珍惜誠信，可以讓我們更加專注于處理自己的事情，也讓事情處理起來更加簡單、高效。
4.關愛生命尊重與關愛生命，是我們職責，也是我們的發(fā)展動力。我們守護自己與家人，快樂工作，享受生活;我們珍視生存的世界，崇尚和諧、友善的人際關系;熱愛優(yōu)美、多彩的人居環(huán)境。