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IL-4細(xì)胞因子
英文名稱(chēng):總訪問(wèn):165
國(guó)產(chǎn)/進(jìn)口:國(guó)產(chǎn)半年訪問(wèn):4
產(chǎn)地/品牌:廈門(mén)/逸漠IMMOCELL產(chǎn)品類(lèi)別:細(xì)胞生物學(xué)試劑
規(guī)       格:10ug/瓶 最后更新:2024-2-20
貨       號(hào):IMF-I04
CAS   號(hào):
參考報(bào)價(jià):900
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▍Backgound

Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.

▍Source

Recombinant Human IL-4 is expressed from Escherichia coli. It contains AA His 25 - Ser 153. Predicted molecular mass 17.7 kDa. This protein carries a polyhistidine tag at the N-terminus.

Recombinant Human IL-4 is expressed from Escherichia coli

▍Endotoxin

Less than 1.0 EU per μg by the LAL method.

▍AA Sequence

HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS

▍Purity

Purity >90% as determined by SDS-PAGE.

▍Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

▍Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

▍Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

▍SDS-PAGE

image.png Human IL-4, His Tag on SDS-PAGE under reducing (R) condition

Human IL-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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