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IL-7細(xì)胞因子
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國(guó)產(chǎn)/進(jìn)口:國(guó)產(chǎn)半年訪問:2
產(chǎn)地/品牌:廈門/逸漠IMMOCELL產(chǎn)品類別:細(xì)胞生物學(xué)試劑
規(guī)       格:10ug/瓶 最后更新:2024-2-20
貨       號(hào):IMF-I07
CAS   號(hào):
參考報(bào)價(jià):1200
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▍Backgound

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.

▍Source

Recombinant Human IL-7 is expressed from Escherichia coli. It contains AA Asp 26 - His 177. Predicted molecular mass 20.2 kDa. This protein carries a polyhistidine tag at the N-terminus.

Recombinant Human IL-7 is expressed from Escherichia coli

▍Endotoxin

Less than 1.0 EU per μg by the LAL method.

▍AA Sequence

DCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH

▍Purity

Purity >90% as determined by SDS-PAGE.

▍Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

▍Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

▍Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

▍SDS-PAGE

Human IL-7, His Tag on SDS-PAGE under reducing (R) condition

Human IL-7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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