product information

Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.

native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Western blot (WB), ELISA (ELISA)

collection of antibodies to fish proteins

to be added when available

application information

1: 5 000 (WB), 1: 5 000 on sole serum (ELISA)

ca. 200 kDa

Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream) 

n. a.

no confirmed exceptions from predicted reactivity known in the moment

the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.

Guzman et al. (2008)  Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.