| product information
| | background | | LRIG1 protein Q96JA1 is coded by a gene on a chromosome band 3p14.3, this region is known to be deleted in various human cancers. LRIG1 is considered to be a a tumour suppressor gene in humans. LRIG1 is an integral cell-surface membrane protein that is expressed by specific cells in various human tissues and that its 143-kDa form might be cleaved into 111-kDa and 32-kDa fragments. The LRIG1 protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor. | | immunogen | | KLH-conjugated synthetic peptide; the peptide corresponds to part of the cytoplasmic tail (C-terminal part) of the protein | | antibody format | | rabbit; | polyclonal; | | affinity purified IgG in PBS pH 7.4, BSA 1mg/ml; | lyophilized | | | quantity | | 100 µg | - for reconstitution add90,9 µl of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | Western blot (WB) | | related products | | AS06 156 | anti-LRIG1 hen antibody | | additional information | | Peptide used to elicit Lrig1 antibody is not conserved in Lrig3 protein. | |
application information
|
| recommended dilution | | 1 µg/ml (WB) |
| expected | apparent MW | | 143 kDa |
| confirmed reactivity | | human, mice |
| predicted reactivity | | human |
| not reactive in | | no confirmed exceptions from predicted reactivity known in the moment |
| additional information | | immunoblotting demonstrated LRIG1 protein in tissue lysates from normal human prostate, mammary epithelial cells, ileum, stomach, lung, and cerebral cortex |
| selected references | | Ledda et al. (2008). Lrig1 is an endogenous inhibitor of Ret receptor tyrosine kinase activation, downstream signaling, and biological responses to GDNF. J. Neruosci. 28(1):2196-07. |
application information
western blot
5 µg of total protein from LRIG1-transfected COS-7 cells extracted with lysis buffer (100 mM Tris-HCl pH 7.5 1M, 300mM NaCl 5M, 2% Triton X-100) were separated on 3-8 % Tris acetate SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% dried milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at the indicated concentrations for 1h at RT with agitation.The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:5 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.
5 µg of total protein from LRIG1-transfected COS-7 cells extracted with lysis buffer (100mM Tris-HCl pH 7.5 1M, 300mM NaCl 5M, 2% Triton X-100) were separated on 3-8 % Tris acetate SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% dried milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody [1ug/uL] at a dilution of 1:3500, 1:1600, 1:800 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (abio-equip.com
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