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產(chǎn)品信息
產(chǎn)品簡介
2-脫氧-D-葡萄糖（2-Deoxy-D-glucose, 2-DG），一種不可代謝的葡萄糖類似物，通過作用于己糖激酶（糖酵解的限速步驟）來抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P，后者不能被磷酸葡萄糖異構(gòu)酶進(jìn)一步代謝，進(jìn)而引起2-DG-P在細(xì)胞內(nèi)的累積和細(xì)胞內(nèi)ATP的耗盡。體外缺氧模型中，2-DG導(dǎo)致細(xì)胞周期抑制和細(xì)胞死亡，誘導(dǎo)自噬，增加ROS生成和活化AMPK。體內(nèi)動物模型中阻斷腫瘤細(xì)胞生長。
產(chǎn)品特性
1） CAS NO：154-17-6
2） 化學(xué)名：(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol
3） 同義名：2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脫氧葡萄糖；2-去氧-D-葡萄糖；D-2-脫氧葡萄糖；
4） 分子式：C6H12O5
5） 分子量：164.16
6） 外觀：白色至類白色固體
7） 純度：>98%
8） 溶解性：溶于DMSO（20mg/ml）、H2O（30mg/ml）、PBS, pH 7.2 （10mg/ml）
保存與運輸方法
保存：2-8℃避光干燥保存，至少2年有效。
運輸：室溫運輸。
注意事項
1） 本品儲存液置于≤-20℃避光保存，盡量避免反復(fù)凍融。通常情況下，-20℃至少一個月穩(wěn)定，-80℃至少6個月穩(wěn)定。
2） 本品僅用作科研用途，絕不可用于臨床診斷或治療藥物，絕不可用做食品或藥品。
3)為了您的安全和健康，請穿實驗服并戴一次性手套操作。
儲存液配制
使用方法【源自文獻(xiàn)，僅作參考】
文獻(xiàn)1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689
體外研究（細(xì)胞實驗）：
細(xì)胞類型（Cell types）：MCF-7 cells
實驗方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm.
實驗結(jié)果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).
體內(nèi)研究（動物模型）：
動物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.
實驗結(jié)果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.
文獻(xiàn)2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277
體內(nèi)研究（動物模型）：
動物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age
配制方法（Formulation）：2-DGwasdiluted insaline.
實驗方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).
實驗結(jié)果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.
| 貨號 | 產(chǎn)品名稱 | 規(guī)格 | 價格/元 |
| ICE1201-0001G | 2-Deoxy-D-glucose 2-脫氧-D-葡萄糖 | 1g | 567 |
| ICE1201-0005G | 2-Deoxy-D-glucose 2-脫氧-D-葡萄糖 | 5g | 2298 |
| ICE1201-0025G | 2-Deoxy-D-glucose 2-脫氧-D-葡萄糖 | 25g | 詢價 |
2-脫氧-D-葡萄糖（2-Deoxy-D-glucose, 2-DG），一種不可代謝的葡萄糖類似物，通過作用于己糖激酶（糖酵解的限速步驟）來抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P，后者不能被磷酸葡萄糖異構(gòu)酶進(jìn)一步代謝，進(jìn)而引起2-DG-P在細(xì)胞內(nèi)的累積和細(xì)胞內(nèi)ATP的耗盡。體外缺氧模型中，2-DG導(dǎo)致細(xì)胞周期抑制和細(xì)胞死亡，誘導(dǎo)自噬，增加ROS生成和活化AMPK。體內(nèi)動物模型中阻斷腫瘤細(xì)胞生長。
產(chǎn)品特性
1） CAS NO：154-17-6
2） 化學(xué)名：(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol
3） 同義名：2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脫氧葡萄糖；2-去氧-D-葡萄糖；D-2-脫氧葡萄糖；
4） 分子式：C6H12O5
5） 分子量：164.16
6） 外觀：白色至類白色固體
7） 純度：>98%
8） 溶解性：溶于DMSO（20mg/ml）、H2O（30mg/ml）、PBS, pH 7.2 （10mg/ml）
保存與運輸方法
保存：2-8℃避光干燥保存，至少2年有效。
運輸：室溫運輸。
注意事項
1） 本品儲存液置于≤-20℃避光保存，盡量避免反復(fù)凍融。通常情況下，-20℃至少一個月穩(wěn)定，-80℃至少6個月穩(wěn)定。
2） 本品僅用作科研用途，絕不可用于臨床診斷或治療藥物，絕不可用做食品或藥品。
3)為了您的安全和健康，請穿實驗服并戴一次性手套操作。
儲存液配制
| 質(zhì)量 濃度 | 1mg | 5mg | 10mg |
| 1mM | 6.0916 mL | 30.4581 mL | 60.9162mL |
| 5mM | 1.2183mL | 6.0916 mL | 12.1832mL |
| 10mM | 0.6092 mL | 3.0458 mL | 6.0916 mL |
| 50mM | 0.1218 mL | 0.6092 mL | 1.2183 mL |
文獻(xiàn)1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689
體外研究（細(xì)胞實驗）：
細(xì)胞類型（Cell types）：MCF-7 cells
實驗方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm.
實驗結(jié)果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).
體內(nèi)研究（動物模型）：
動物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.
實驗結(jié)果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.
文獻(xiàn)2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277
體內(nèi)研究（動物模型）：
動物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age
配制方法（Formulation）：2-DGwasdiluted insaline.
實驗方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).
實驗結(jié)果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.
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