Description:

Rabbit polyclonal antibody

  Background:

The light-harvesting complex (LHC) functions as a light receptor; it captures and delivers excitation energy to photosystems. Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with PSII in plants and algae. CP29 facilitates the State 1 to State 2 transition, where State I is induced by excess photosystem I (PSI) light and State 2 is induced by excess photosystem II (PSII) light.

  Synonyms:

Lhcb4, Chlorophyll a-b binding protein CP29

  Immunogen:

KLH-conjugated synthetic peptide of Lhcb4 derived from Arabidopsis thaliana AT5G01530.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

31 / 30 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Brassica napus, Brassica rapa,and 80-99% homologues with the sequence inCucumis sativus, Populus trichocarpa, Solanum lycopersicum, Nicotiana tabacum, Gossypium raimondii, Vitis vinifera,v Zea mays, Oryza sativa Japonica Group, Glycine max.
For more species homologues information, please contact tech support at tech@phytoab.com.

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.