Description:

Rabbit polyclonal antibody

  Background:

HHL1 is a chloroplast protein and participates in photodamage repair of PSII under high light. Mutants without HHL1 mutants show hypersensitivity to high light and drastically decreased PSII photosynthetic activity. HHL1 associates with the PSII core monomer complex through direct interaction with PSII core proteins CP43 and CP47, and also interacts with LQY1, which functions in the repair and reassembly of PSII.

  Synonyms:

HHL1, HYPERSENSITIVE TO HIGH LIGHT 1

  Immunogen:

KLH-conjugated synthetic peptide of HHL1 derived from Arabidopsis thaliana AT1G67700.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

26 / 24 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 2.5 μg and 5 μg of chlorophyll, respectively. 
3 is thylakoid membrane protein from hhl1 mutant of Arabidopsis thaliana leaf containing 5 μg of chlorophyll. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.