Product Information Description: Rabbit polyclonal antibody Background: Arabidopsis thaliana has two genes (atpC1, atpC2) coding for gamma subunits of chloroplast ATP synthase. The atpC1 and atpC2 were cloned and sequenced. Synonyms: AtpC Immunogen: KLH-conjugated synthetic peptide of AtpC1 derived from Arabidopsis thaliana AT4G04640. Form: Lyophilized Quantity: 150 µg Purification: Protein A purified Reconstitution: Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube". Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution. Shipping: The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. Application Information Recommended Dilution: Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user. Expected/apparent MW: 41 / 36 kDa Confirmed Reactivity: Arabidopsis thaliana Predicted Reactivity: Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. Chinensis, and 80-99% homologues with the sequence in Populus trichocarpa, Medicago truncatula, Nicotiana tabacum, Glycine max, Zea mays. For more species homologues information, please contact tech support at tech@phytoab.com. Application Example 1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. Electrophoresis: 15% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection: using chemiluminescence substrate and image were captured with CCD camera.