Description:

Rabbit polyclonal antibody

  Background:

The D1/D2 (PsbA/PsbD) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors. D2 is needed for assembly of a stable PSII complex.

  Synonyms:

PsbD

  Immunogen:

KLH-conjugated synthetic peptide derived from DE-loop of D2 subunit in Arabidopsis thaliana ATCG00270.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39.5 / 33 kDa(Arabidopsis thaliana), 35 kDa(Synechocystis sp. PCC 6803)

  Confirmed Reactivity: 

Arabidopsis thaliana, Synechocystis sp. PCC 6803

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Oryza sativa Indica Group, Glycine max, Solanum tuberosum, Cucumis sativus, Sorghum bicolor, Triticum aestivum, Hordeum vulgare subsp. Vulgare, Zea mays, Nicotiana tabacum, Setaria viridis, Spinacia oleracea, Populus trichocarpa, Vitis vinifera, Chlamydomonas reinhardtii, Leymus chinensis,and 80-99% homologues with the sequence in Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.