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                                                              TOP1 Human
                                                              英文名稱:TOP1 Human總訪問:226
                                                              國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:ENZ-306 最后更新:2024-12-5
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                                                              Catalogue Number ENZ-306
                                                              Synonyms DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.
                                                              Introduction DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.
                                                              In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name.
                                                              Description DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 Dalton (excluding glycosilation) 102 kDa total mass. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
                                                              Source Sf9 insect cells.
                                                              Physical Appearance Sterile Filtered clear solution.
                                                              Formulation TOP1 is supplied in 16mM HEPES buffer pH-7.5, 400mM sodium chloride, and 20% glycerol.
                                                              Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
                                                              Store, frozen at -20°C for longer periods of time.
                                                              Avoid multiple freeze-thaw cycles.
                                                              Purity Greater than 95% as determined by SDS-PAGE.
                                                              Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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