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Catalogue Number | ENZ-352 |
Synonyms | Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG. |
Description | E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases). |
Source | Escherichia Coli strain that carries the UNG gene from E.coli. |
Physical Appearance | Sterile Filtered colorless solution. |
Formulation | UNG solution in 10mM Tris-Hcl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol. |
Stability | Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
Applications | Glycosilase mediated single nucleotide polymorphism detection (GMPD). Site-directed mutagenesis. As a probe for protein-DNA interaction studies. Rapid and efficient cloning of PCR products. Elimination carry-over contamination in PCR. |
Specific Activity | The Specific Activity was found to be 5U/µl. |
Unit Definition |
1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C. |
10X UNG Reaction Buffer | 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA. |
Reaction Conditions | 1X UNG Reaction Buffer, incubate at 37°C. UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. |
Inhibition and Inactivation |
Inactivated by heating at 95°C for 10min. Enzye activity is partially restored at temperatures lower than 55°C. |
Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |