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IDH1
英文名稱:IDH1總訪問:536
國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
規(guī)       格:ENZ-289 最后更新:2024-12-5
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Catalogue Number ENZ-289
Synonyms Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD.
Description ICDH is isolated from Saccharomyces Cerevisiae.
The ICDH is purified by proprietary chromatographic techniques.
Source Saccharomyces Cerevisiae.
Physical Appearance Sterile Filtered clear solution.
Formulation One ml of solution (1mg/340µl) contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.
Stability ICDH although stable at 15°C for 1 week should be stored between 2°C-8°C.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA)
Please avoid freeze-thaw cycles.
Purity Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity The specific activity was found to be 119 U/mg.
Unit Definition One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Introduction Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
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