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Catalogue Number | ENZ-289 |
Synonyms | Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD. |
Description | ICDH is isolated from Saccharomyces Cerevisiae. The ICDH is purified by proprietary chromatographic techniques. |
Source | Saccharomyces Cerevisiae. |
Physical Appearance | Sterile Filtered clear solution. |
Formulation | One ml of solution (1mg/340µl) contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1. |
Stability | ICDH although stable at 15°C for 1 week should be stored between 2°C-8°C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA) Please avoid freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Biological Activity | The specific activity was found to be 119 U/mg. |
Unit Definition | One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25°C, pH 7.5). |
Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Introduction | Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. |