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                                                              FTCD Human
                                                              英文名稱:FTCD Human總訪問:354
                                                              國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:ENZ-302 最后更新:2024-12-5
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                                                              Catalogue Number ENZ-302
                                                              Synonyms Formiminotransferase-cyclodeaminase, FTCD, LCHC1, LC-1.
                                                              Introduction Formiminotransferase cyclodeaminase is an enzyme which catalyzes the conversion of formiminoglutamateand tetrahydrofolateinto formiminotetrahydrofolateand glutamate.
                                                              Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker).
                                                              Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.
                                                              Description Formiminotransferase Cyclodeaminase Human Recombinant (also called liver cytosol type 1) produced in SF9, is a glycosylated, polypeptide chain having a molecular mass of 59,749 Dalton.
                                                              The FTCD is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
                                                              Source Sf9 insect cells.
                                                              Formulation FTCD is supplied in 16mM HEPES buffer pH-7.6, 240mM sodium chloride, and 20% glycerol.
                                                              Immunological functions 1. Binds IgG-type human auto-antibodies.
                                                              2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
                                                              Applications Western-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient
                                                              sera.
                                                              Coating concentration 0.35-0.7 µg/ml (depending on the type of ELISA plate and coating buffer).
                                                              Suitable for biotinylation and iodination.
                                                              Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
                                                              Store, frozen at -20°Cfor longer periods of time.
                                                              Avoid multiple freeze-thaw cycles.
                                                              Purity Greater than 95% as determined by SDS-PAGE.
                                                              Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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