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CKMT1 Human
英文名稱:CKMT1 Human總訪問:328
國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:5
產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
規(guī)       格:CKI-271 最后更新:2024-12-5
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Catalogue Number CKI-271
Synonyms Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.
Introduction Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Description CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by proprietary chromatographic techniques.
Source Pichia Pastoris.
Physical Appearance Sterile Filtered colourless liquid formulation.
Formulation CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.
Stability CKMT1 although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by
a) Analysis by RP-HPLC
b) Analysis by SDS-PAGE
Biological Activity The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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