Myeloid Leukemia Factor 1 Human Recombinant Shipping:Ice Packs Synonyms Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1. Introduction Myeloid leukemia factor 1 (MLF1) is a member of the MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. MLF1 hinders the erythropoietin-induced erythroid terminal differentiation by averting cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 generally functions in multi-potent progenitor cells, and its dysregulation may be to some extent responsible for leukemogenesis. Translocations between the MLF1 gene and nucleophosmin are linked to myelodysplastic syndrome and acute myeloid leukemia. Description MLF1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 288 amino acids (1-268 a.a.) and having a molecular mass of 32.8kDa (Molecular weight on SDS-PAGE will appear higher). The MLF1 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered colorless solution. Formulation The MLF1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 5mM DTT and 200mM NaCl. Stability MLF1 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. Purity Greater than 85.0% as determined by SDS-PAGE. Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDS>

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