Centrin-1 Human Recombinant Shipping:Ice Packs Synonyms Centrin EF-hand protein 1, calcium binding protein, Caltractin isoform 2, CETN. Introduction CETN1 is an EF-hand type Ca2+-binding protein. CETN1 is localized to the centrosome of interphase cells, and reorganizes the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. CETN1 has a vital part in the determination of centrosome position and isolation, and in the course of microtubule separating. Description CETN1 Human Recombinant produced E. coli is a single polypeptide chain containing 221 amino acids (1-172) and having a molecular mass of 22.1kDa.
CETN1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Source E.coli. Physical Appearance Sterile Filtered colorless solution. Formulation The CETN1 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol. Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Amino acid sequence MGSSHHHHHH SSGLVPRGSH MGSHMASGFK KPSAASTGQK RKVAPKPELT EDQKQEVREA FDLFDVDGSG TIDAKELKVA MRALGFEPRK EEMKKMISEV DREGTGKISF NDFLAVMTQK MSEKDTKEEI LKAFRLFDDD ETGKISFKNL KRVANELGEN LTDEELQEMI DEADRDGDGE VNEEEFLRIM KKTSLY >

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