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b NGF Human, HEK
英文名稱:b NGF Human, HEK總訪問:236
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產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
規(guī)       格:CYT-079 最后更新:2024-12-5
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Catalogue Number CYT-079
Synonyms Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Introduction NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
Description Beta-NGF Human Recombinant produced in HEK cells is a non-glycosylated non-disulfide linked homodimer, having a total molecular weight of 13kDa.
The b-NGF is purified by proprietary chromatographic techniques.
Source HEK.
Physical Appearence Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The b-NGF was lyophilized from 1mg/ml in 1xPBS.
Solubility It is recommended to reconstitute the lyophilized b-NGF in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution b-NGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95% as obsereved by SDS-PAGE.
Biological Activity The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 1.5-8ng/ml.
Usage ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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