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| 銷售商： 北京啟維益成科技有限公司 | 查看該公司所有產(chǎn)品 >> |
| Catalogue Number | CHM-350 |
| Synonyms | CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298. |
| Introduction | Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy. |
| Description | CXCL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.8 kDa. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered white lyophilized powder. |
| Formulation |
The CXCL4 protein was lyophilized after extensive dialysis against 50mM Tris-HCl pH 8.0 and 150mM NaCl buffer. |
| Solubility |
It is recommended to reconstitute the lyophilized CXCL4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized CXCL4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL4 should be stored at 4°C between 2-7 days and for future use below -18°C. |
| Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence |
The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu-Asp. |
| Biological Activity |
The ED50 of CXCL4 as determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts was found to be 5-15 µg/ml. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. |
