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                                                              當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 細(xì)胞生物學(xué)試劑 > IGF1 Gilthead Seabream
                                                              



                                                              

                                                              
 
	
                                                              

		
                                                              
			
                                                              
			  
                                                              
			    
				
				IGF1 Gilthead Seabream
					
			    
				
					
					
                                                              
						
                                                              
							英文名稱：IGF1 Gilthead Seabream總訪問：420		
						
                                                              
						
                                                              
							國產(chǎn)/進(jìn)口：進(jìn)口半年訪問：8 
						
                                                              
						
                                                              
							產(chǎn)地/品牌：prospec產(chǎn)品類別：細(xì)胞生物學(xué)試劑
						
                                                              
						
                                                              
							規(guī)       格：CYT-295

	
	最后更新：2024-12-5
						
                                                              
						
                                                              
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Catalogue Number 
CYT-295 
                                                              

                                                              
Synonyms 
Somatomedin C, IGF-I, IGFI. 
                                                              

                                                              
Introduction 
The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003). 
                                                              

                                                              
Description 
Insulin-Like Growth Factor-IGilthead SeabreamRecombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7545.4 Dalton, the predicted pI=7.72.
                                                              IGF-1 is purified by proprietary chromatographic techniques. 
                                                              

                                                              
Source 
Escherichia Coli. 
                                                              

                                                              
Physical Appearance 
Sterile Filtered White lyophilized (freeze-dried) powder. 
                                                              

                                                              
Formulation 
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. 
                                                              

                                                              
Solubility 
It is recommended to reconstitute the lyophilized IGF-1 in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 
                                                              

                                                              
Stability 
Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
                                                              For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles. 
                                                              

                                                              
Purity 
Greater than 98.0% as determined by:
                                                              (a) Analysis by SEC-HPLC.
                                                              (b) Analysis by SDS-PAGE. 
                                                              

                                                              
Amino acid sequence 
The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ser-Pro-Glu-Thr-Leu-Cys-Gly-Ala-Glu. 
                                                              

                                                              
Biological Activity 
Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active. 
                                                              

                                                              
Protein content 
Somatomedin C quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.60 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 
                                                              2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard. 
                                                              

                                                              
Usage 
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
                                                              >
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