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                                                              FGF 18 Human
                                                              英文名稱:FGF 18 Human總訪問:299
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                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:CYT-120 最后更新:2024-12-5
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                                                              Catalogue Number CYT-120
                                                              Synonyms Fibroblast growth factor 18, FGF-18, zFGF5, FGF18.
                                                              Introduction Fibroblast growth factor 18 (FGF18) is a member of the large FGF family which has at least 23 members. FGF18 is a heparin binding growth factor with a core 120 amino acid FGF domain which allows for a common tertiary structure. FGFs are expressed in the course of the embryonic development and in restricted adult tissues. FGF-18 is an indispensable regulator of long bone and calvarial development. FGF-18 signals via FGFR 1c, 2c, 3c, and 4.
                                                              Description FGF-18 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 20.1kDa.
                                                              The FGF-18 is purified by proprietary chromatographic techniques.
                                                              Source Escherichia Coli.
                                                              Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation FGF-18 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
                                                              Solubility It is recommended to reconstitute the lyophilized FGF-18 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles.
                                                              Purity Greater than 95.0% as determined by:
                                                              (a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Amino Acid Sequence AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSR.
                                                              Biological Activity The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is < 0.5ng/ml, corresponding to a specific activity of > 2.0×106 units/mg.
                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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