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                                                              GM CSF K9
                                                              英文名稱:GM CSF K9總訪問:251
                                                              國產(chǎn)/進口:進口半年訪問:2
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細胞生物學(xué)試劑
                                                              規(guī)       格:CYT-724 最后更新:2024-12-5
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                                                              Catalogue Number CYT-724
                                                              Synonyms CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.
                                                              Introduction GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
                                                              GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
                                                              Description GMCSF k9 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques.
                                                              Source Escherichia Coli.
                                                              Physical Appearence Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation GMCSF was lyophilized after extensive dialysis against 1xPBS pH 7.4.
                                                              Solubility It is recommended to reconstitute the lyophilized GMCSF in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability

                                                              Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles.

                                                              Purity Greater than 96.0% as determined by
                                                              1. Analysis by RP-HPLC.
                                                              2. Analysis by SDS-PAGE.
                                                              Amino Acid Sequence APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE
                                                              GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN
                                                              INFKSFKENL KDFLFNIPFD CWKPVKK.
                                                              Biological Activity The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml.
                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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