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                                                              IL 6 Human, CHO
                                                              英文名稱:IL 6 Human, CHO總訪問:283
                                                              國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:CYT-274 最后更新:2024-12-5
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                                                              Catalogue Number CYT-274
                                                              Synonyms IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
                                                              Introduction IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
                                                              Description Interleukin-6 Human Recombinant produced in CHO is a single, glycosylated polypeptide chain containing 185 amino acids and migrates at 22 kDa as a glycosilated protein on SDS-PAGE.
                                                              The IL6 is purified by proprietary chromatographic techniques.
                                                              Source Chinese Hamster Ovarian Cells.
                                                              Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation Lyophilized from a solution containing Phosphate- Buffered Saline pH-7.4.
                                                              Solubility It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
                                                              For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles.
                                                              Purity Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Amino Acid Sequence APVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM.
                                                              Biological Activity The activity was measured in a cell prolifiration assay using B9 cells. The ED50 for this effect is 0.008-0.02ng/ml corresponding to a Specific Activity of 50-125MIU/mg.
                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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