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| Catalogue Number | CYT-211 | ||||||||
| Synonyms | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. | ||||||||
| Introductionn | IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. | ||||||||
| Description | Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The IL-4 is purified by proprietary chromatographic techniques. | ||||||||
| Source | Escherichia Coli. | ||||||||
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | ||||||||
| Formulation | Lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. | ||||||||
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||||||
| Stability | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | ||||||||
| Purity | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | ||||||||
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp. | ||||||||
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. | ||||||||
| Protein content | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. | ||||||||
| References |
1. Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation1 |
