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                                                              IL 3 Rat
                                                              英文名稱:IL 3 Rat總訪問:2327
                                                              國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:2
                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:CYT-383 最后更新:2024-12-5
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                                                              Catalogue Number CYT-383
                                                              Synonyms MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
                                                              Introduction Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells.
                                                              IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
                                                              Description Interleukin-3 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 16 kDa.
                                                              The IL-3 is purified by proprietary chromatographic techniques.
                                                              Source Escherichia Coli.
                                                              Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation Lyophilized from a 1mg/ml solution without any additives.
                                                              Solubility It is recommended to reconstitute the lyophilized Interleukin 3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.
                                                              For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles.
                                                              Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
                                                              (b) Analysis by SDS-PAGE.
                                                              Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Asp-Arg-Gly.
                                                              Biological Activity The ED50= 3-10 ng/ml corresponding to a specific activity of 100,000-333,334IU/mg determined by the dose dependent proliferation of murine NFS-60 cells.
                                                              Protein content Protein quantitation was carried out by two independent methods
                                                              1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

                                                              2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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