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                                                              IL 12 Rat
                                                              英文名稱:IL 12 Rat總訪問:296
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                                                              產(chǎn)地/品牌:prospec產(chǎn)品類別:細胞生物學(xué)試劑
                                                              規(guī)       格:CYT-390 最后更新:2024-12-5
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                                                              Catalogue Number CYT-390
                                                              Synonyms NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
                                                              Introduction IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.
                                                              Description Interleukin-12 Rat Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
                                                              The IL-12 is purified by proprietary chromatographic techniques.
                                                              Source Baculovirus.
                                                              Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
                                                              Formulation The protein (1mg/ml) was lyophilized with no additives.
                                                              Solubility It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
                                                              Stability Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.
                                                              For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
                                                              Please prevent freeze-thaw cycles.
                                                              Purity Greater than 95.0% as determined by SDS-PAGE.
                                                              Amino acid sequence The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
                                                              Biological Activity ED50 range = 0.01-0.05 ng/ml corresponding to a specific activity of 20-100MIU/mg as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated mouse splenocytes that have been costimulated with PMA. Optimal concentration for individual application should be determined by a dose response assay.
                                                              Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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