Description:

Rabbit polyclonal antibody

  Background:

Fructose 1,6-biphosphate aldolase (FBA) is aldolase superfamily protein. It is a key enzyme in plants, which is involved not only in glycolysis and gluconeogenesis in the cytoplasm, but also in the Calvin cycle in plastids. In the current study, eight FBA family genes (AtFBA1-8) were identified and analyzed in Arabidopsis thaliana: FBA1 (AT2G21330), FBA2 (AT4G38970), FBA3 (AT2G01140), FBA4 (AT5G03690), FBA5 (AT4G26530), FBA6 (AT2G36460), FBA7 (AT4G26520), FBA8 (AT3G52930).

  Synonyms:

FBA8, ATFBA8, FRUCTOSE-BISPHOSPHATE ALDOLASE 8

  Immunogen:

KLH-conjugated synthetic peptide of FBA8 derived from Arabidopsis thaliana AT3G52930.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Medicago truncatula.
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 18 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg cytosolic protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.